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Addgene inc e kowarz
E Kowarz, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/psbbi+gh/pSBbi-GH+(Plasmid+%2360514)/pm41559400-340-21-23
Average 92 stars, based on 9 article reviews
e kowarz - by Bioz Stars, 2026-10
92/100 stars

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Polymerase Chain Reaction:

Article Title: Barcoding of small extracellular vesicles with CRISPR-gRNA enables comprehensive, subpopulation-specific analysis of their biogenesis and release regulators
Article Snippet: .. CD9 was PCR-amplified from pDB965 (PhCMV-CD9-nluc-pAbGH) using oKK620 and oKK621, digested with SfiI and cloned into the corresponding sites (SfiI) of pSBbi-GH (addgene #605142). ..

Article Title: Barcoding of small extracellular vesicles with CRISPR-gRNA enables comprehensive, subpopulation-specific analysis of their biogenesis and release regulators
Article Snippet: .. CD63 was PCR-amplified from pDB305 using oKK127 and oKK128, digested with SfiI and cloned into the corresponding sites (SfiI) of pSBbi-GH (addgene #605141). .. The GTIS sequence in the linker can be digested by KpnI-HF/NheI-HF to allow insertion of the gene to be fused to CD63 This work pKK60 Constitutive expression of CD63-dCas9. dCas9 was PCR-amplified from lenti-EF1αdCas9-KRAB-Puro (addgene #993726) using oKK129 and oKK130 (excluding Nterminal nuclear localization signal (NLS), KRPAATKKAGQAKKKK), digested with KpnI-HF/NheI-HF and cloned into the corresponding sites (KpnI-HF/NheI-HF) of pKK59.

Article Title: Barcoding of small extracellular vesicles with CRISPR-gRNA enables comprehensive, subpopulation-specific analysis of their biogenesis and release regulators
Article Snippet: Horlbeck et al, addgene #848322 pKK47 Constitutive expression of CD63-MS2. .. CD63-MS2 was PCR-amplified from pSA_Exo010 (unpublished, encoding MS2 reported by Wroblewska et al3) from using oKK29 and oKK103, digested with SfiI and cloned into the corresponding sites (SfiI) of pSBbi-GH (addgene #605141). .. This work pKK49 Constitutive expression of mock MS2-gRNA for lentiviral transduction. gRNA scaffold bearing MS2 coat protein-binding motif was PCR-amplified from sgRNA(MS2) cloning backbone (addgene #614244) using oKK109 and oKK105, digested with BlpI/XhoI and cloned into the corresponding sites (BlpI/XhoI) of pCRISPRia-v2 (addgene #848322).

Clone Assay:

Article Title: Barcoding of small extracellular vesicles with CRISPR-gRNA enables comprehensive, subpopulation-specific analysis of their biogenesis and release regulators
Article Snippet: .. CD9 was PCR-amplified from pDB965 (PhCMV-CD9-nluc-pAbGH) using oKK620 and oKK621, digested with SfiI and cloned into the corresponding sites (SfiI) of pSBbi-GH (addgene #605142). ..

Article Title: Barcoding of small extracellular vesicles with CRISPR-gRNA enables comprehensive, subpopulation-specific analysis of their biogenesis and release regulators
Article Snippet: .. CD63 was PCR-amplified from pDB305 using oKK127 and oKK128, digested with SfiI and cloned into the corresponding sites (SfiI) of pSBbi-GH (addgene #605141). .. The GTIS sequence in the linker can be digested by KpnI-HF/NheI-HF to allow insertion of the gene to be fused to CD63 This work pKK60 Constitutive expression of CD63-dCas9. dCas9 was PCR-amplified from lenti-EF1αdCas9-KRAB-Puro (addgene #993726) using oKK129 and oKK130 (excluding Nterminal nuclear localization signal (NLS), KRPAATKKAGQAKKKK), digested with KpnI-HF/NheI-HF and cloned into the corresponding sites (KpnI-HF/NheI-HF) of pKK59.

Article Title: Barcoding of small extracellular vesicles with CRISPR-gRNA enables comprehensive, subpopulation-specific analysis of their biogenesis and release regulators
Article Snippet: Horlbeck et al, addgene #848322 pKK47 Constitutive expression of CD63-MS2. .. CD63-MS2 was PCR-amplified from pSA_Exo010 (unpublished, encoding MS2 reported by Wroblewska et al3) from using oKK29 and oKK103, digested with SfiI and cloned into the corresponding sites (SfiI) of pSBbi-GH (addgene #605141). .. This work pKK49 Constitutive expression of mock MS2-gRNA for lentiviral transduction. gRNA scaffold bearing MS2 coat protein-binding motif was PCR-amplified from sgRNA(MS2) cloning backbone (addgene #614244) using oKK109 and oKK105, digested with BlpI/XhoI and cloned into the corresponding sites (BlpI/XhoI) of pCRISPRia-v2 (addgene #848322).

Plasmid Preparation:

Article Title: Cell-specific bioorthogonal tagging of glycoproteins.
Article Snippet: Restriction enzymes were from New England Biolabs. .. The plasmids pSBtet-GH and pSBbi-GH were a gift from Eric Kowarz (Addgene plasmid #60498; RRI- D:Addgene_60498, and Addgene plasmid # 60514; RRID:Addgene_60514)43. pTriEx6-His-GST-3C-MCS was an in-house construct modified from pTriEx-6 (Supplementary Data 4)19. .. The plasmid pCMV(CAT)T7-SB100 (Addgene plasmid #34879; Addgene: pCMV(CAT)T7-SB100; RRI- D:Addgene_34879) was a kind gift from Zsuzsanna Izsvak42.

Construct:

Article Title: Cell-specific bioorthogonal tagging of glycoproteins.
Article Snippet: Restriction enzymes were from New England Biolabs. .. The plasmids pSBtet-GH and pSBbi-GH were a gift from Eric Kowarz (Addgene plasmid #60498; RRI- D:Addgene_60498, and Addgene plasmid # 60514; RRID:Addgene_60514)43. pTriEx6-His-GST-3C-MCS was an in-house construct modified from pTriEx-6 (Supplementary Data 4)19. .. The plasmid pCMV(CAT)T7-SB100 (Addgene plasmid #34879; Addgene: pCMV(CAT)T7-SB100; RRI- D:Addgene_34879) was a kind gift from Zsuzsanna Izsvak42.

Modification:

Article Title: Cell-specific bioorthogonal tagging of glycoproteins.
Article Snippet: Restriction enzymes were from New England Biolabs. .. The plasmids pSBtet-GH and pSBbi-GH were a gift from Eric Kowarz (Addgene plasmid #60498; RRI- D:Addgene_60498, and Addgene plasmid # 60514; RRID:Addgene_60514)43. pTriEx6-His-GST-3C-MCS was an in-house construct modified from pTriEx-6 (Supplementary Data 4)19. .. The plasmid pCMV(CAT)T7-SB100 (Addgene plasmid #34879; Addgene: pCMV(CAT)T7-SB100; RRI- D:Addgene_34879) was a kind gift from Zsuzsanna Izsvak42.



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